ABSTRACT
PURPOSE: This study detects SARS-CoV-2 in the ocular surface through one-step reverse-transcription droplet digital PCR (one-step RT-ddPCR) and evaluates the possibility of the ocular surface as a possible transmission route. METHODS: A single-center prospective observational study was designed to investigate the viral loads in ocular surface. Specimens including the conjunctival swabs, nasopharyngeal swabs and blood were synchronously collected at a single time point for all COVID-19 patients. SARS-CoV-2 loads in nasopharyngeal swabs were tested by real-time polymerase chain reaction (PCR); the blood samples and conjunctival swabs were tested by real-time PCR and one-step RT-ddPCR. RESULTS: Sixty-eight COVID-19 patients confirmed by nasopharyngeal real-time PCR were recruited. In the single time point test, 40 cases showed positive SARS-CoV-2 detection in either the blood, tears, or nasopharynx, of which four cases were triple-positive, 10 were dual-positive, and 26 were single-positive. The positive rate of nasopharyngeal swab real-time PCR test was 22.1% (15/68). The positive rate of blood and conjunctival swabs by one-step RT-ddPCR was 38.2% (26/68) and 25% (17/68), respectively, whereas real-time PCR was all negative. Positive conjunctival swabs were significantly correlated with positive nasopharyngeal swabs (P = 0.028). The sampling lags from illness onset to sampling day in 3 out of 4 triple-positive patients and in 9 out of 10 dual-positive patients were respectively less than 9 days and less than 20 days. CONCLUSION: Our results indicate that the positive rate of SARS-CoV-2 on the ocular surface is much higher than expected. Transmission possibility through the ocular surface may be greatly underestimated.
ABSTRACT
This study aimed to examine the combined effect of voluntary exercise and screen time during the first wave of the coronavirus disease 2019 (COVID-19) restrictions on subsequent grip strength among health and sports science students in Japan. The participants performed grip strength tests and had their weight assessed on 21 October 2020. Furthermore, they completed an online survey between 21 October and 28 October 2020, which included participants’current age, height, voluntary exercise, and screen time during the first wave of the COVID-19 pandemic (April and May 2020). In total, 380 participants provided complete data (mean age ±standard deviation [SD]: 18.8 ±0.6 years;233 males). Our results revealed that 87.6% of male students and 84.4% of female students performed voluntary exercise >1 day/week during the first wave of the pandemic. Moreover, 21.5% of male students and 23.1% of female students had >8 h/day of screen time. A trend toward greater grip strength was observed for both male and female students with high voluntary exercise and low screen time. In particular, grip strength (p < 0.05) was significantly greater in male students with high voluntary exercise and low screen time than those with low voluntary exercise and high screen time. In conclusion, this study revealed that during the first wave of COVID-19 restrictions, a combination of high voluntary exercise and low screen time positively affected the subsequent grip strength of health and sports science students.